技术原理与实验步骤

采用噬菌体介导的同源重组进行基因敲除:首先构建同源交换位点(AES),随后将其整合到结核分枝杆菌噬菌体基 因组中,获得噬菌粒(phasmid);将噬菌粒导入耻垢分枝杆菌,获得整合有同源交换位点的重组噬菌体,经体外扩 增获得高滴度的重组噬菌体,对结核分枝杆菌进行转染;将转染后的结核分枝杆菌涂布到含潮霉素抗性的固体培养基 上,37°C培养 4-5 周,挑取单克隆,通过 PCR 等方式进行验证。

 

结核分枝杆菌基因敲除菌株现货

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Rv编号基因名蛋白名
Rv0007-membrane protein
Rv0009ppiAiron-regulated peptidyl-prolyl cis-trans isomerase PpiA
Rv0010c-membrane protein
Rv0032bioF28-amino-7-oxononanoate synthase
Rv0035fadD34fatty-acid--CoA ligase FadD34
Rv0044c-oxidoreductase
Rv0067c-transcriptional regulator
Rv0072-glutamine ABC transporter permease
Rv0077c-oxidoreductase
Rv0078-transcriptional regulator
Rv0084hycDformate hydrogenlyase HycD
Rv0085hycPhydrogenase HycP
Rv0088-polyketide cyclase/dehydrase
Rv0099fadD10fatty-acid--CoA ligase FadD10
Rv0131cfadE1acyl-CoA dehydrogenase FadE1
Rv0133-GCN5-like N-acetyltransferase
Rv0135c-transcriptional regulator
Rv0138-hypothetical protein Rv0138
Rv0151cPE1PE family protein PE1
Rv0153cptbBphosphotyrosine protein phosphatase
Rv0154cfadE2acyl-CoA dehydrogenase FadE2
Rv0159cPE3PE family protein PE3
Rv0166fadD5fatty-acid--CoA ligase FadD5
Rv0176-Mce associated transmembrane protein
Rv0184-hypothetical protein Rv0184
Rv0187-O-methyltransferase
Rv0190-hypothetical protein Rv0190
Rv0195-two component transcriptional regulator
Rv0199-membrane protein
Rv0203-hypothetical protein Rv0203
Rv0204c-transmembrane protein
Rv0210-hypothetical protein Rv0210
Rv0211pckAphosphoenolpyruvate carboxykinase
Rv0215cfadE3Probable acyl-CoA dehydrogenase FadE3
Rv0222echA1enoyl-CoA hydratase EchA1
Rv0231fadE4acyl-CoA dehydrogenase FadE4
Rv0242cfabG43-oxoacyl-ACP reductase FabG
Rv0243fadA2acetyl-CoA acetyltransferase FadA
Rv0244cfadE5acyl-CoA dehydrogenase FadE5
Rv0250c-hypothetical protein Rv0250c
Rv0254ccobUbifunctional cobinamide kinase/cobinamide phosphate guanylyltransferase
Rv0256cPPE2PPE family protein PPE2
Rv0264c-hypothetical protein Rv0264c
Rv0267narUnitrite extrusion protein NarU
Rv0271cfadE6acyl-CoA dehydrogenase FadE6
Rv0272c-hypothetical protein Rv0272c
Rv0275c-transcriptional regulator
Rv0279cPE_PGRS4PE-PGRS family protein PE_PGRS4
Rv0294tamtrans-aconitate methyltransferase
Rv0295c-hypothetical protein Rv0295c
Rv0311-hypothetical protein Rv0311
Rv0313-hypothetical protein Rv0313
Rv0314c-membrane protein
Rv0315-beta-1,3-glucanase
Rv0319pcppyrrolidone-carboxylate peptidase
Rv0320-hypothetical protein Rv0320
Rv0332-hypothetical protein Rv0332
Rv0335cPE6PE family protein PE6
Rv0349-hypothetical protein Rv0349
Rv0354cPPE7PPE family protein PPE7
Rv0355cPPE8PPE family protein PPE8
Rv0358-hypothetical protein Rv0358
Rv0362mgtEMg2+ transport transmembrane protein MgtE
Rv0370c-oxidoreductase
Rv0381c-hypothetical protein Rv0381c
Rv0387c-Conserved hypothetical protein
Rv0390-hypothetical protein Rv0390
Rv0392cndhANADH dehydrogenase NdhA
Rv0405pks6membrane bound polyketide synthase
Rv0413mutT38-oxo-dGTP diphosphatase
Rv0419lpqMlipoprotein peptidase LpqM
Rv0420c-transmembrane protein
Rv0451cmmpS4membrane protein MmpS4
Rv0463-membrane protein
Rv0464c-hypothetical protein Rv0464c
Rv0472c-HTH-type transcriptional regulator
Rv0475hbhAheparin binding hemagglutinin HbhA
Rv0494-HTH-type transcriptional regulator
Rv0498-hypothetical protein Rv0498
Rv0519c-membrane protein
Rv0535-S-methyl-5\'-thioadenosine phosphorylase
Rv0536galE3UDP-glucose 4-epimerase GalE
Rv0549cvapC3ribonuclease VapC3
Rv0551cfadD8fatty-acid--CoA ligase FadD8
Rv0573cpncB2nicotinic acid phosphoribosyltransferase PncB2
Rv0580c-hypothetical protein Rv0580c
Rv0598cvapC27ribonuclease VapC27
Rv0612-hypothetical protein Rv0612
Rv0631crecCexonuclease V subunit gamma RecC
Rv0665vapC8ribonuclease VapC8
Rv0672fadE8acyl-CoA dehydrogenase FadE8
Rv0698-hypothetical protein Rv0698
Rv0699-hypothetical protein Rv0699
Rv0739-hypothetical protein Rv0739
Rv0740-hypothetical protein Rv0740
Rv0748vapB31antitoxin VapB31
Rv0750-hypothetical protein Rv0750
Rv0762c-hypothetical protein Rv0762c
Rv0770-oxidoreductase
Rv0774c-hypothetical protein Rv0774c
Rv0787-hypothetical protein Rv0787
Rv0793-monooxygenase
Rv0801-hypothetical protein Rv0801
Rv0804-hypothetical protein Rv0804
Rv0816cthiXthioredoxin ThiX
Rv0826-hypothetical protein Rv0826
Rv0832PE_PGRS12PE-PGRS family protein PE_PGRS12
Rv0841-transmembrane protein
Rv0843-dehydrogenase
Rv0846c-oxidase
Rv0863-hypothetical protein Rv0863
Rv0878cPPE13PPE family protein PPE13
Rv0880-HTH-type transcriptional regulator
Rv0905echA6enoyl-CoA hydratase EchA6
Rv0906-hypothetical protein Rv0906
Rv0915cPPE14PPE family protein PPE14
Rv0916cPE7PE family protein PE7
Rv0918-hypothetical protein Rv0918
Rv0925c-hypothetical protein Rv0925c
Rv0927c-oxidoreductase
Rv0959-hypothetical protein Rv0959
Rv0969ctpVcopper-exporting ATPase
Rv0971cechA7enoyl-CoA hydratase EchA7
Rv0974caccD2acetyl-/propionyl-CoA carboxylase subunit beta
Rv0979c-hypothetical protein Rv0979c
Rv0998-acetyltransferase
Rv1001arcAarginine deiminase
Rv1003-rRNA small subunit methyltransferase I
Rv1027ckdpEtranscriptional regulator KdpE
Rv1035c-Probable transposase (fragment)
Rv1037cesxIESAT-6 like protein EsxI
Rv1039cPPE15PPE family protein PPE15
Rv1045-hypothetical protein Rv1045
Rv1061-hypothetical protein Rv1061
Rv1069c-hypothetical protein Rv1069c
Rv1070cechA8enoyl-CoA hydratase EchA8
Rv1071cechA9enoyl-CoA hydratase EchA9
Rv1076lipUlipase LipU
Rv1083-hypothetical protein Rv1083
Rv1088a-hypothetical protein Rv1088a
Rv1096-glycosyl hydrolase
Rv1112-GTP-binding protein
Rv1118c-hypothetical protein Rv1118c
Rv1135cPPE16PPE family protein PPE16
Rv1136-Possible enoyl-CoA hydratase
Rv1142cechA10enoyl-CoA hydratase EchA10
Rv1143mcralpha-methylacyl-CoA racemase
Rv1145-transmembrane transport protein
Rv1151c-NAD-dependent protein deacylase
Rv1154c-hypothetical protein Rv1154c
Rv1155a-hypothetical protein Rv1155a
Rv1159A-pterin-4-alpha-carbinolamine dehydratase
Rv1169clipXlipase LipX
Rv1185cfadD21fatty-acid--CoA ligase FadD21
Rv1186c-hypothetical protein Rv1186c
Rv1191-hypothetical protein Rv1191
Rv1193fadD36fatty-acid--CoA ligase FadD36
Rv1204c-hypothetical protein Rv1204c
Rv1207folP2dihydropteroate synthase
Rv1226c-transmembrane protein
Rv1228a-hypothetical protein Rv1228a
Rv1239ccorAmagnesium and cobalt transport transmembrane protein CorA
Rv1243cPE_PGRS23PE-PGRS family protein PE_PGRS23
Rv1250-MFS-type drug transporter
Rv1263amiB2amidase AmiB
Rv1272c-drug ABC transporter ATP-binding protein
Rv1273c-drug ABC transporter ATP-binding protein
Rv1280coppAoligopeptide ABC transporter substrate-binding lipoprotein OppA
Rv1300hemKrelease factor glutamine methyltransferase
Rv1324-thioredoxin
Rv1325cPE_PGRS24PE-PGRS family protein PE_PGRS24
Rv1330cpncB1nicotinic acid phosphoribosyltransferase PncB1
Rv1345mbtMlong-chain-fatty-acid--ACP ligase MbtM
Rv1346mbtNacyl-ACP dehydrogenase MbtN
Rv1347cmbtKlysine N-acetyltransferase MbtK
Rv1362c-membrane protein
Rv1363c-membrane protein
Rv1386PE15PE family protein PE15
Rv1411clprGlipoprotein LprG
Rv1427cfadD12long-chain-fatty-acid--CoA ligase FadD12
Rv1450cPE_PGRS27PE-PGRS family protein PE_PGRS27
Rv1455-hypothetical protein Rv1455
Rv1471trxB1thioredoxin
Rv1474c-transcriptional regulator
Rv1478-peptidoglycan endopeptidase RipB
Rv1489-hypothetical protein Rv1489
Rv1489A-hypothetical protein Rv1489A
Rv1492mutAmethylmalonyl-CoA mutase small subunit
Rv1527cpks5polyketide synthase
Rv1537dinXDNA polymerase IV
Rv1555frdDfumarate reductase membrane anchor subunit
Rv1558-hypothetical protein Rv1558
Rv1566c-hypothetical protein Rv1566c
Rv1591-transmembrane protein
Rv1593c-hypothetical protein Rv1593c
Rv1627c-nonspecific lipid-transfer protein
Rv1638uvrAexcinuclease ABC subunit UvrA
Rv1671-membrane protein
Rv1694tlyA16S/23S rRNA (cytidine-2\'-O)-methyltransferase TlyA
Rv1706cPPE23PPE family protein PPE23
Rv1717-hypothetical protein Rv1717
Rv1729c-S-adenosylmethionine-dependent methyltransferase
Rv1744c-membrane protein
Rv1759cwag22PE-PGRS family protein Wag22
Rv1767-hypothetical protein Rv1767
Rv1768PE_PGRS31PE-PGRS family protein PE_PGRS31
Rv1789PPE26PPE family protein PPE26
Rv1790PPE27PPE family protein PPE27
Rv1791PE19PE family protein PE19
Rv1800PPE28PPE family protein PPE28
Rv1801PPE29PPE family protein PPE29
Rv1808PPE32PPE family protein PPE32
Rv1814erg3membrane-bound C-5 sterol desaturase
Rv1823-hypothetical protein Rv1823
Rv1824-hypothetical protein Rv1824
Rv1833c-haloalkane dehalogenase
Rv1839cvapB13antitoxin VapB13
Rv1840cPE_PGRS34PE-PGRS family protein PE_PGRS34
Rv1847-esterase
Rv1851ureFurease accessory protein UreF
Rv1857modAmolybdate ABC transporter substrate-binding lipoprotein ModA
Rv1860apahypothetical protein Rv1860
Rv1897c-D-tyrosyl-tRNA(Tyr) deacylase
Rv1899clppDlipoprotein LppD
Rv1901cinAcompetence damage-inducible protein CinA
Rv1902cnanTsialic acid-transport integral membrane protein NanT
Rv1903-membrane protein
Rv1915aceAaisocitrate lyase AceAa
Rv1918cPPE35PPE family protein PPE35
Rv1940ribA1riboflavin biosynthesis protein RibA
Rv1941-short-chain type dehydrogenase/reductase
Rv1942cmazF5toxin MazF5
Rv1962cvapC35ribonuclease VapC35
Rv1965yrbE3Bintegral membrane protein
Rv1967mce3BMce family protein Mce3B
Rv1969mce3DMce family protein Mce3D
Rv1978-hypothetical protein Rv1978
Rv1980cmpt64immunogenic protein Mpt64
Rv1986-amino acid transporter
Rv1997ctpFcation transporter ATPase F
Rv2000-hypothetical protein Rv2000
Rv2002fabG320-beta-hydroxysteroid dehydrogenase
Rv2011c-hypothetical protein Rv2011c
Rv2025c-cation efflux system protein
Rv2027cdosTtwo component sensor histidine kinase DosT
Rv2031chspXalpha-crystallin
Rv2039c-sugar ABC transporter permease
Rv2043cpncApyrazinamidase/nicotinamidase PncA
Rv2046lppIlipoprotein LppI
Rv2048cpks12polyketide synthase
Rv2054-hypothetical protein Rv2054
Rv2074-pyridoxamine 5\'-phosphate oxidase
Rv2078-hypothetical protein Rv2078
Rv2082-hypothetical protein Rv2082
Rv2086-hypothetical protein Rv2086
Rv2090-5\'-3\' exonuclease
Rv2097cpafA1901年9月3日
Rv2099cPE21PE family protein PE21 (pseudogene)
Rv2101helZhelicase HelZ
Rv2108PPE36PPE family protein PPE36
Rv2111cpupubiquitin-like protein Pup
Rv2118c-tRNA (adenine(58)-N(1))-methyltransferase
Rv2119-hypothetical protein Rv2119
Rv2123PPE37PPE family protein PPE37
Rv2124cmetHmethionine synthase
Rv2136c-undecaprenyl-diphosphatase
Rv2142AparD2antitoxin ParD2
Rv2146c-transmembrane protein
Rv2162cPE_PGRS38PE-PGRS family protein PE_PGRS38
Rv2173idsA2geranylgeranyl pyrophosphate synthetase IdsA
Rv2177c-transposase
Rv2187fadD15long-chain-fatty-acid--CoA ligase FadD15
Rv2189c-hypothetical protein Rv2189c
Rv2197c-transmembrane protein
Rv2203-membrane protein
Rv2222cglnA2glutamine synthetase
Rv2231ccobCaminotransferase
Rv2234ptpAprotein-tyrosine-phosphatase
Rv2237-hypothetical protein Rv2237
Rv2246kasB3-oxoacyl-ACP synthase 2
Rv2248-hypothetical protein Rv2248
Rv2267c-hypothetical protein Rv2267c
Rv2268ccyp128cytochrome P450 Cyp128
Rv2270lppNlipoprotein LppN
Rv2271-hypothetical protein Rv2271
Rv2272-transmembrane protein
Rv2286c-hypothetical protein Rv2286c
Rv2287yjcENa(+)/H(+) transporter
Rv2290lppOlipoprotein LppO
Rv2295-hypothetical protein Rv2295
Rv2297-hypothetical protein Rv2297
Rv2307A-hypothetical protein Rv2307A
Rv2308a-hypothetical protein Rv2308a
Rv2311-hypothetical protein Rv2311
Rv2325c-hypothetical protein Rv2325c
Rv2333cstpmultidrug resistance protein
Rv2335cysEserine acetyltransferase
Rv2340cPE_PGRS39PE-PGRS family protein PE_PGRS39
Rv2342-hypothetical protein Rv2342
Rv2345-transmembrane protein
Rv2346cesxOESAT-6 like protein EsxO
Rv2347cesxPESAT-6 like protein EsxP
Rv2351cplcAmembrane-associated phospholipase A
Rv2352cPPE38PPE family protein PPE38
Rv2353cPPE39PPE family protein PPE39
Rv2356cPPE40PPE family protein PPE40
Rv2358smtBHTH-type transcriptional regulator SmtB
Rv2382cmbtCpolyketide synthetase
Rv2386a-hypothetical protein Rv2386a
Rv2404clepAGTP-binding protein LepA
Rv2410c-hypothetical protein Rv2410c
Rv2416ceisenhanced intracellular survival protein
Rv2417c-DegV domain-containing protein
Rv2428ahpCalkyl hydroperoxide reductase subunit AhpC
Rv2430cPPE41PPE family protein PPE41
Rv2431cPE25PE family protein PE25
Rv2436rbsKribokinase RbsK
Rv2450crpfEresuscitation-promoting factor RpfE
Rv2458mmuMhomocysteine S-methyltransferase MmuM
Rv2459-MFS-type transporter
Rv2462ctigtrigger factor
Rv2482cplsB2glycerol-3-phosphate acyltransferase
Rv2483cplsCbifunctional L-3-phosphoserine phosphatase/1-acyl-sn-glycerol-3-phosphate acyltransferase
Rv2500cfadE19acyl-CoA dehydrogenase FadE19
Rv2502caccD1acetyl-/propionyl-CoA carboxylase subunit beta
Rv2519PE26PE family protein PE26
Rv2525c-hypothetical protein Rv2525c
Rv2529-hypothetical protein Rv2529
Rv2536-transmembrane protein
Rv2551c-hypothetical protein Rv2551c
Rv2566-hypothetical protein Rv2566
Rv2573-2-dehydropantoate 2-reductase
Rv2575-membrane protein
Rv2579dhaAhaloalkane dehalogenase
Rv2590fadD9fatty-acid--CoA ligase FadD9
Rv2606csnzPpyridoxine biosynthesis protein
Rv2608PPE42PPE family protein PPE42
Rv2615cPE_PGRS45PE-PGRS family protein PE_PGRS45
Rv2620c-transmembrane protein
Rv2621c-transcriptional regulator
Rv2632c-hypothetical protein Rv2632c
Rv2634cPE_PGRS46PE-PGRS family protein PE_PGRS46
Rv2637dedAtransmembrane protein DedA
Rv2646-integrase
Rv2663-hypothetical protein Rv2663
Rv2679echA15enoyl-CoA hydratase EchA15
Rv2683-hypothetical protein Rv2683
Rv2692ceoCTRK system potassium uptake protein CeoC
Rv2694c-hypothetical protein Rv2694c
Rv2700-hypothetical protein Rv2700
Rv2709-transmembrane protein
Rv2714-hypothetical protein Rv2714
Rv2729c-integral membrane protein
Rv2731-hypothetical protein Rv2731
Rv2741PE_PGRS47PE-PGRS family protein PE_PGRS47
Rv2749-hypothetical protein Rv2749
Rv2767c-membrane protein
Rv2770cPPE44PPE family protein PPE44
Rv2776c-oxidoreductase
Rv2792c-resolvase
Rv2820c-CRISPR type III-associated RAMP protein Csm4
Rv2823c-CRISPR-associated protein Cas10/Csm1
Rv2827c-hypothetical protein Rv2827c
Rv2828A-hypothetical protein Rv2828A
Rv2840c-hypothetical protein Rv2840c
Rv2849ccobOcob(I)alamin adenosyltransferase
Rv2859c-glutamine amidotransferase
Rv2864c-penicillin-binding lipoprotein
Rv2873mpt83cell surface lipoprotein
Rv2886c-resolvase
Rv2887-HTH-type transcriptional regulator
Rv2899cfdhDformate dehydrogenase accessory protein FdhD
Rv2939papA5phthiocerol/phthiodiolone dimycocerosyl transferase
Rv2946cpks1polyketide synthase
Rv2947cpks15polyketide synthase
Rv2954c-hypothetical protein Rv2954c
Rv3004cfp6low molecular weight protein antigen 6
Rv3007c-oxidoreductase
Rv3013-hypothetical protein Rv3013
Rv3017cesxQESAT-6 like protein EsxQ
Rv3020cesxSESAT-6 like protein EsxS
Rv3022APE29PE family protein PE29
Rv3040c-hypothetical protein Rv3040c
Rv3047c-hypothetical protein Rv3047c
Rv3055-TetR family transcriptional regulator
Rv3057c-short chain alcohol dehydrogenase/reductase
Rv3058c-TetR family transcriptional regulator
Rv3061cfadE22acyl-CoA dehydrogenase FadE22
Rv3078habhydroxylaminobenzene mutase
Rv3082cvirSHTH-type transcriptional regulator VirS
Rv3087-diacyglycerol O-acyltransferase
Rv3088tgs4diacyglycerol O-acyltransferase
Rv3089fadD13long chain-fatty-acid--CoA ligase FadD13
Rv3097clipYtriacylglycerol lipase Lip
Rv3098A-PemK-like protein
Rv3104c-transmembrane protein
Rv3125cPPE49PPE family protein PPE49
Rv3132cdevStwo component sensor histidine kinase DevS
Rv3133cdevRtwo component transcriptional regulator DevR
Rv3136PPE51PPE family protein PPE51
Rv3139fadE24acyl-CoA dehydrogenase FadE24
Rv3141fadB4NADPH quinone oxidoreductase FadB
Rv3143-response regulator
Rv3144cPPE52PPE family protein PPE52
Rv3154nuoJNADH-quinone oxidoreductase subunit J
Rv3159cPPE53PPE family protein PPE53
Rv3164cmoxR3methanol dehydrogenase transcriptional regulator MoxR
Rv3167c-TetR family transcriptional regulator
Rv3170aofHflavin-containing monoamine oxidase
Rv3190c-hypothetical protein Rv3190c
Rv3196-hypothetical protein Rv3196
Rv3196A-hypothetical protein Rv3196A
Rv3199cnudCNADH pyrophosphatase
Rv3207c-hypothetical protein Rv3207c
Rv3233c-Possible triacylglycerol synthase (diacylglycerol acyltransferase)
Rv3241c-hypothetical protein Rv3241c
Rv3263-DNA methylase
Rv3289c-transmembrane protein
Rv3294c-hypothetical protein Rv3294c
Rv3301cphoY1phosphate transport system transcriptional regulator PhoY
Rv3308pmmBphosphomannomutase PmmB
Rv3309cuppuracil phosphoribosyltransferase
Rv3310sapMacid phosphatase
Rv3312A-pilin
Rv3318sdhAsuccinate dehydrogenase flavoprotein subunit
Rv3334-MerR family transcriptional regulator
Rv3335c-integral membrane protein
Rv3343cPPE54PPE family protein PPE54
Rv3344cPE_PGRS49PE-PGRS family protein PE_PGRS49
Rv3345cPE_PGRS50PE-PGRS family protein PE_PGRS50
Rv3347cPPE55PPE family protein PPE55
Rv3373echA18enoyl-CoA hydratase
Rv3374echA18.1Probable enoyl-CoA hydratase (fragment) EchA18.1
Rv3376-phosphatase
Rv3382c-4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Rv3392ccmaA1cyclopropane mycolic acid synthase CmaA
Rv3395A-membrane protein
Rv3409cchoDcholesterol oxidase
Rv3415c-hypothetical protein Rv3415c
Rv3425PPE57PPE family protein PPE57
Rv3426PPE58PPE family protein PPE58
Rv3429PPE59PPE family protein PPE59
Rv3439c-hypothetical protein Rv3439c
Rv3448eccD4ESX-4 secretion system protein EccD4
Rv3466-hypothetical protein Rv3466
Rv3478PPE60PE family protein PPE60
Rv3483c-hypothetical protein Rv3483c
Rv3491-hypothetical protein Rv3491
Rv3506fadD17long-chain-fatty-acid--CoA ligase FadD17
Rv3508PE_PGRS54PE-PGRS family protein PE_PGRS54
Rv3510c-hypothetical protein Rv3510c
Rv3511PE_PGRS55PE-PGRS family protein PE_PGRS55
Rv3512PE_PGRS56PE-PGRS family protein PE_PGRS56
Rv3514PE_PGRS57PE-PGRS family protein PE_PGRS57
Rv3516echA19enoyl-CoA hydratase EchA19
Rv3532PPE61PPE family protein PPE61
Rv3534chsaF4-hydroxy-2-oxovalerate aldolase
Rv3539PPE63PPE family protein PPE63
Rv3545ccyp125steroid C26-monooxygenase
Rv3548c-short-chain type dehydrogenase/reductase
Rv3550echA20enoyl-CoA hydratase EchA20
Rv3557c-HTH-type transcriptional regulator KstR2
Rv3563fadE32acyl-CoA dehydrogenase FadE32
Rv3566cnatarylamine N-acetyltransferase
Rv3574kstRHTH-type transcriptional regulator KstR
Rv3595cPE_PGRS59PE-PGRS family protein PE_PGRS59
Rv3619cesxVESAT-6 like protein EsxV
Rv3620cesxWESAT-6 like protein EsxW
Rv3621cPPE65PPE family protein PPE65
Rv3622cPE32PE family protein PE32
Rv3641cficcell filamentation protein Fic
Rv3652PE_PGRS60PE-PGRS family-related protein PE_PGRS60
Rv3653PE_PGRS61PE-PGRS family-related protein PE_PGRS61
Rv3671c-serine protease
Rv3674cnthendonuclease III
Rv3677c-beta lactamase
Rv3678c-hypothetical protein Rv3678c
Rv3685ccyp137cytochrome P450 Cyp137
Rv3686c-hypothetical protein Rv3686c
Rv3690-membrane protein
Rv3691-hypothetical protein Rv3691
Rv3692moxR2methanol dehydrogenase transcriptional regulator MoxR
Rv3705c-hypothetical protein Rv3705c
Rv3732-hypothetical protein Rv3732
Rv3737-transmembrane protein
Rv3738cPPE66PPE family protein PPE66
Rv3739cPPE67PPE family protein PPE67
Rv3746cPE34PE family protein PE34
Rv3755c-hypothetical protein Rv3755c
Rv3774echA21enoyl-CoA hydratase EchA21
Rv3785-hypothetical protein Rv3785
Rv3803cfbpDMPT51/MPB51 antigen
Rv3812PE_PGRS62PE-PGRS family protein PE_PGRS62
Rv3822-hypothetical protein Rv3822
Rv3837c-phosphoglycerate mutase
Rv3849espRESX-1 transcriptional regulator EspR
Rv3857c-membrane protein
Rv3865espFESX-1 secretion-associated protein EspF
Rv3868eccA1ESX-1 secretion system protein EccA1
Rv3870eccCa1ESX-1 secretion system protein EccCa
Rv3871eccCb1ESX-1 secretion system protein EccCb
Rv3873PPE68PPE family protein PPE68
Rv3874esxBESAT-6-like protein EsxB
Rv3876espIESX-1 secretion-associated protein EspI
Rv3878espJESX-1 secretion-associated protein EspJ
Rv3881cespBESX-1 secretion-associated protein EspB
Rv3885ceccE2ESX-2 secretion system protein EccE
Rv3890cesxCESAT-6 like protein EsxC
Rv3891cesxDESAT-6 like protein EsxD
Rv3892cPPE69PPE family protein PPE69
Rv3893cPE36PE family protein PE36
Rv3894ceccC2ESX-2 type VII secretion system protein EccC
Rv3895ceccB2ESX-2 secretion system protein EccB
Rv3897c-hypothetical protein Rv3897c
Rv3898c-hypothetical protein Rv3898c
Rv3900c-hypothetical protein Rv3900c
Rv3922c-membrane protein insertion efficiency factor

 

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更新:
2018.06. 结核分枝杆菌基因敲除菌株现货共计210株
2018.08. 结核分枝杆菌基因敲除菌株现货共计275株
2018.08. 结核分枝杆菌基因敲除菌株现货共计344株
2018.10. 结核分枝杆菌基因敲除菌株现货共计449株
2018.11. 结核分枝杆菌基因敲除菌株现货共计477株
2018.11. 结核分枝杆菌基因敲除菌株现货共计517株